Batch foam separation of a soluble protein

Batch foam separation of a soluble protein.

Removal of protein dissolved in water by batch foam separation was conducted with using ovalbumin (OA) as a model protein in the light of wastewater treatment reducing organic loading. The removal efficiency had a maximum value near the i.e.p. of OA (pH 4.6); thus, most experiments were conducted at pH 4.6. Typical experimental conditions; superficial gas velocity, U(g): 1.97 x 10(-2)-5.37 x 10...

Selective Foam Separation of Binary Protein Solution

A fundamental study about the selective foam separation of protein mixture was carried out. A solution containing two proteins, Ovalbumin (OA) and Lysozyme (LZ), and an anionic surfactant, Sodium Dodecyle Sulfate (SDS), was adjusted to pH 6.0 which refered to an intermediate state between the isoelectric points of the proteins. The solution was processed by continuous foam separation. The resul...

Optimization of EnBase Fed-Batch Cultivation to Improve Soluble Fraction Ratio of α-Luffin Ribosome Inactivating Protein

Background: The increase of the protein expression via ribosomal manipulation is one of the suggested cellular mechanisms involved in EnBase fed-batch mode of cultivation. However, this system has not been implemented for cytotoxic proteins.Objectives: Here, the expression pattern of α-Luffin, a ribosome inactivation protein (RIP) with an innate toxicity,...

Foam separation of microbial cells.

Batch foam separation has been employed to separate Saccharomyces carlsbergensis cells from their broth without the use of any external surface-active agent. A model has been developed to predict the foamate cell concentration as well as the variation of cell concentration in the bulk liquid in the foam column as a function of time. The model assumes a linear equilibrium relation between the ce...

Batch Separation of Styrene/Ethyl Benzene/Water Dispersions